Please use this identifier to cite or link to this item: doi:10.22028/D291-38248
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Title: Activity-guided purification reveals quercetin as the most efficient cholinesterase inhibitor in wild strawberry (Fragaria vesca L.) and apricot (Prunus armeniaca L.) fruit extract
Author(s): Zapp, Josef
Kamila Borowiec, Dominik Szwajgier,
Language: English
Title: Emirates Journal of Food and Agriculture
Volume: 31
Issue: 5
Pages: 386-394
Publisher/Platform: UAE University
Year of Publication: 2019
Free key words: Apricot
Cholinesterase inhibitors
Wild strawberry
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: Cholinesterase (ChE) inhibitors are commonly approved for the Alzheimer’s disease therapy which should restore the original levels of acetylcholine in the brain. In this work, a suitable purification procedure of ChE inhibitors from wild strawberry (Fragaria vesca L.) and apricot (Prunus armeniaca L.) fruit is presented. Activity-guided purification was based on ultrafiltration (0.2 μm → 5 kDa → 3 kDa) of water extract from fruit followed by preparative chromatography (Sephadex LH-20 and HPLC-C18) and analytical HPLC-C18. At every stage of purification, anti-ChE activity was measured in each fraction using Ellman’s colorimetric method. The most active ChE inhibitor purified from both fruits was quercetin and the chemical structure was confirmed by infrared (IR) and proton nuclear magnetic resonance (1H-NMR) spectroscopies. The fast purification procedure can be used for isolating phenolic ChE inhibitors from other fruits from the Rosaceae family.
DOI of the first publication: 10.9755/ejfa.2019.v31.i5.1957
URL of the first publication:
Link to this record: urn:nbn:de:bsz:291--ds-382485
ISSN: 2079-0538
Date of registration: 28-Nov-2022
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Pharmazie
Professorship: NT - Prof. Dr. Alexandra K. Kiemer
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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