Description of Streptomyces explomaris sp. nov., isolated from the coastal soil rhizosphere of Juniperus excelsa and reclassification of Streptomyces libani as a later heterotypic synonym of Streptomyces nigrescens

Abstract

The strain Je 1-4T was isolated from the coastal rhizosphere soil of Juniperus excelsa M. Bieb. (Crimean Peninsula). Phylogenetic studies based on the 16S rRNA gene sequence revealed that Je 1-4T is phylogenetically close to Streptomyces libani subsp. libani NBRC 13452T (JCM 4322) and Streptomyces nigrescens NBRC 12894T (DSM 40276) with sequence similarities of 99.86 to 99.93%. The genome of strain Je 1-4T consisted of a linear chromosome with a size of 8.9 Mbp and a G+C content of 70.9mol%. Digital DNA–DNA hybridization (dDDH) analysis showed that Je 1-4T is distinct from both S. libani subsp. libani and S. nigrescens (dDDH values of 66.6% and 66.7%, respectively), while the latter two strains likely represent the same species (dDDH value of 92.0%). The predominant fatty acids in the strains were iso-C16:0 , anteiso-C17:0 and anteiso-C15:0 , and the major menaqui nones were MK9 H6 and MK9 H8. Based on these genomic and phenotypic data, strain Je 1-4T represents a novel species of Streptomyces, for which the name Streptomyces explomaris sp. nov. is proposed. The type strain is Je 1-4T (=DSM 117375T =LMG 33490T ). Additionally, we propose that S. libani subsp. libani Baldacci and Grein 1966 (Approved Lists 1980) is a later heterotypic synonym of S. nigrescens (Sveshnikova 1957) Pridham et al. 1958 (Approved Lists 1980).